Goat anti-Mouse IgG (H+L), Biotin-AffiniPure
货号:PMK-015-095M/S
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价格:
240.00
规格:
100ul
1ml
货期:
现货
本产品4℃运输,保存于4℃,保质期 12个月
Details
产品详情
  • Applications

    WB,IF,ELISA,IHC,Fc

  • Species Cross-Reactivity

    Mouse

  • Molecular Weight

    N/A

  • Isotype

    IgG1

  • Storage Buffer & Condition

    PBS, pH 7.4, 15mg/ml Bovine Serum Albumin as Stablizer and  50% Glycerol. Store at -20°C. 

    Do not aliquot the antibody.


  • Suggested Dilution Range

    WB: 1:10000-1:50000  

    IHC  1:500 - 1:5000  

    IF:1:200-1:1000


  • Purity

    The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to 

    agarose beads


  • Antibody Specificity

    Based on immunoelectrophoresis and/or EL1SA,the antibody reacts with whole molecule mouse IgG. lt also

    reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-

    immunoglobulin serum proteins.The antibody may cross-react with immunoglobulins from other species.


  • Expiration date

    Store at -20°C. Avoid repeated freeze / thaw cycles.The expiration date may be extended if test results are

    acceptable for the intended use.


  • Note

    For in vitro research use only,not for diagnostic or therapeutic use.This product is not a medical device.

Instruction manual/COA download
说明书/COA下载
Published literature
已发表文献
  • 更新中...

Product Q&A FAQ
产品问答FAQ
  • Western Blot(WB)实验中有很多杂带咋回事?

    答:可能的原因有:

    1、目的蛋白有多个修饰位点,本身可以呈现多条带,建议查阅文献或进行生物信息学分析,获得蛋白序列的修饰位点信息,通过去修饰确定蛋白实际大小;

    2、样本处理过程中目的蛋白发生降解,建议加入蛋白酶抑制剂;样本处理时在冰上操作;

    3、杂蛋白多,建议处理目的蛋白;

    4、抗体特异性不强,建议使用特异性强的抗体;

    5、抗体孵育时间过久,建议减少抗体孵育时间;

    6、二抗与抗原有交叉反应,建议选择合适的封闭物;

    7、二聚体或多聚体存在,建议增加蛋白质变性过程及强度;

    8、底物显色与曝光时间过长,建议缩短显色及曝光的时间。


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