GST-Tag Rabbit Polyclonal Antibody
货号:PMK107M/S
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价格:
580.00
规格:
50ul
100ul
货期:
现货
本产品4℃运输,保存于4℃,保质期 12个月。
Details
产品详情
  • Applications

    WB

  • Species Cross-Reactivity

    N/A

  • Molecular Weight

    N/A

  • Isotype

    IgG

  • Storage Buffer & Condition

    PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C. 

    Do not aliquot the antibody.


  • Recommended dilutions

    WB: 1:5,000 

  • Specificity

    The GST tag antibody can recognize C-terminal, internal, and N-terminal GST fusion proteins.

  • Background

    The glutathione S-transferase (GST, previously known as ligandins) family of enzymes are composed of many cytosolic, 

    mitochondrial, and microsomal proteins. GSTs are present in eukaryotes and in prokaryotes, where they catalyze a 

    variety of reactions and accept endogenous and xenobiotic substrates.GST tag antibody can be helpful in detecting the 

    fusion protein during purification as well as the cleavage of GST from the protein of interest. GST tag antibody has wide 

    applications that could include your research on GST proteins or GST fusion recombinant proteins.


Instruction manual/COA download
说明书/COA下载
Published literature
已发表文献
  • 更新中...

Product Q&A FAQ
产品问答FAQ
  • Western Blot(WB)实验中有很多杂带咋回事?

    答:可能的原因有:

    1、目的蛋白有多个修饰位点,本身可以呈现多条带,建议查阅文献或进行生物信息学分析,获得蛋白序列的修饰位点信息,通过去修饰确定蛋白实际大小;

    2、样本处理过程中目的蛋白发生降解,建议加入蛋白酶抑制剂;样本处理时在冰上操作;

    3、杂蛋白多,建议处理目的蛋白;

    4、抗体特异性不强,建议使用特异性强的抗体;

    5、抗体孵育时间过久,建议减少抗体孵育时间;

    6、二抗与抗原有交叉反应,建议选择合适的封闭物;

    7、二聚体或多聚体存在,建议增加蛋白质变性过程及强度;

    8、底物显色与曝光时间过长,建议缩短显色及曝光的时间。


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