β-actin Mouse Monoclonal Antibody(5B7)-HRP conjugated
货号:PMK058M/S
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价格:
800.00
规格:
50ul
100ul
货期:
现货
本产品4℃运输,保存于4℃,保质期 12个月。
Details
产品详情
  • Applications

    WB, IP

  • Species Cross-Reactivity

    H, R, M, Mk, Dg, Ch, Hm, Rb, Pg, Sh

  • Molecular Weight

    43KD

  • Isotype

    IgG1

  • Storage Buffer & Condition

    PBS, pH 7.4, containing 0.02% Kathon as Preservative and 50% Glycerol. Store at -20°C. Do not aliquot the antibody

  • Recommended dilutions

    WB: 1:5000  

    IHC: 1:100-500 


  • Specificity

    Antibodycandetectsendogenous β-Actin protein.

  • Alternative Names

    actin antibody, b-actin antibody, beta-actin antibody

  • Background

    β-actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and 

    tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against β-actin are useful 

    as loading controls for Western Blotting. However it should be noted that levels of β-actin may not be stable in certain cells. 

    For example, expression of β-actin in adiposetissue is very low and therefore β-actin should not be used as loading control for 

    these tissues.


Instruction manual/COA download
说明书/COA下载
Published literature
已发表文献
  • 更新中...

Product Q&A FAQ
产品问答FAQ
  • Western Blot(WB)实验中有很多杂带咋回事?

    答:可能的原因有:

    1、目的蛋白有多个修饰位点,本身可以呈现多条带,建议查阅文献或进行生物信息学分析,获得蛋白序列的修饰位点信息,通过去修饰确定蛋白实际大小;

    2、样本处理过程中目的蛋白发生降解,建议加入蛋白酶抑制剂;样本处理时在冰上操作;

    3、杂蛋白多,建议处理目的蛋白;

    4、抗体特异性不强,建议使用特异性强的抗体;

    5、抗体孵育时间过久,建议减少抗体孵育时间;

    6、二抗与抗原有交叉反应,建议选择合适的封闭物;

    7、二聚体或多聚体存在,建议增加蛋白质变性过程及强度;

    8、底物显色与曝光时间过长,建议缩短显色及曝光的时间。


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