WB, IP
H, R, M, Mk, Dg, Ch, Hm, Rb, Pg, Sh
43KD
IgG1
PBS, pH 7.4, containing 0.02% Kathon as Preservative and 50% Glycerol. Store at -20°C. Do not aliquot the antibody
WB: 1:5000
IHC: 1:100-500
Antibodycandetectsendogenous β-Actin protein.
actin antibody, b-actin antibody, beta-actin antibody
β-actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and
tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against β-actin are useful
as loading controls for Western Blotting. However it should be noted that levels of β-actin may not be stable in certain cells.
For example, expression of β-actin in adiposetissue is very low and therefore β-actin should not be used as loading control for
these tissues.
1. Yao X ,Shengbo J ,Miao H , et al.Chromatin conformation of human oral epithelium can identify orofacial cleft missing functional variants[J].International Journal of Oral Science,2022,14(1):43-43. (IF 24.897)
2. Shiyu L ,Qi Z ,Songhang L , et al.Tetrahedral framework nucleic acids‐based delivery promotes intracellular transfer of healing peptides and accelerates diabetic would healing[J].Cell Proliferation,2022,55(9):e13279-e13279. (IF 8.755)
3. Konghuai W ,Lu Z ,Hanqing M , et al.Intercellular mitochondrial transfer alleviates pyroptosis in dental pulp damage.[J].Cell proliferation,2023,56(9):e13442-e13442. (IF 8.5)
4. Hui L ,Yuxuan L ,Wei F , et al.Fusobacterium nucleatum triggers proinflammatory cell death via Z-DNA binding protein 1 in apical periodontitis[J].Cell Communication and Signaling,2022,20(1):196-196. (IF 7.525)
5. Sun Y ,Chen Y ,Zhao H , et al.Lactate-driven type I collagen deposition facilitates cancer stem cell-like phenotype of head and neck squamous cell carcinoma[J].iScience,2024,27(4):109340-. (IF 4.6)
6. Huang D ,Li Y ,Han J , et al.Xbp1 promotes odontoblastic differentiation through modulating mitochondrial homeostasis.[J].FASEB journal : official publication of the Federation of American Societies for Experimental Biology,2024,38(7):e23600-e23600. (IF 4.4)
7. Huanyan Z ,Yao X ,Jiahao H , et al.Phosphorylation of ATF2 promotes odontoblastic differentiation via intrinsic HAT activity.[J].Journal of genetics and genomics = Yi chuan xue bao,2023,50(7):497-510. (IF 5.9)
8. Xin W ,MingJing H ,XiaoJie C , et al.Glaucocalyxin A impairs tumor growth via amplification of the ATF4/CHOP/CHAC1 cascade in human oral squamous cell carcinoma.[J].Journal of ethnopharmacology,2022,290115100-115100. (IF 5.195)
9. Zhou L ,Shi W ,Zhang X , et al.Injectable Tannin-Containing Hydroxypropyl Chitin Hydrogel as Novel Bioactive Pulp Capping Material Accelerates Repair of Inflamed Dental Pulp.[J].Biomolecules,2024,14(9):1129-1129. (IF 4.8)
10. Liu H ,Liu X Y ,Fan W , et al.Metformin switches cell death modes to soothe the apical periodontitis via ZBP1.[J].FASEB journal : official publication of the Federation of American Societies for Experimental Biology,2024,38(5):e23549-e23549. (IF 4.4)
11. Min C ,Siyang Z ,Siyu C , et al.Pharmacological inhibition of SMYD2 protects against cisplatin-induced renal fibrosis and inflammation[J].Journal of Pharmacological Sciences,2023,153(1):38-45. (IF 3.5)
12. Tian-Qi M ,Nan-Nan C ,Rong-Cheng X , et al.Indocyanine green-loaded platelet activated by photodynamic and photothermal effects for selective control of wound repair[J].Photodiagnosis and Photodynamic Therapy,2024,45103945-. (IF 3.3)
Gao Y ,Lin Y ,Li Y , et al.Interplay of RUNX2 and KLF4 in initial commitment of odontoblast differentiation.[J].Journal of cellular biochemistry,2024,125(7): (IF 3)
答：可能的原因有：
1、目的蛋白有多个修饰位点，本身可以呈现多条带，建议查阅文献或进行生物信息学分析，获得蛋白序列的修饰位点信息，通过去修饰确定蛋白实际大小；
2、样本处理过程中目的蛋白发生降解,建议加入蛋白酶抑制剂；样本处理时在冰上操作；
3、杂蛋白多，建议处理目的蛋白；
4、抗体特异性不强，建议使用特异性强的抗体；
5、抗体孵育时间过久，建议减少抗体孵育时间；
6、二抗与抗原有交叉反应，建议选择合适的封闭物；
7、二聚体或多聚体存在，建议增加蛋白质变性过程及强度；
8、底物显色与曝光时间过长，建议缩短显色及曝光的时间。
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