WB, IHC，IF
H, R, M, Mk, Dg, C, Hm, Rb, Sh
55KD
IgG1
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.Store at -20°C.
Do not aliquot the antibody.
WB: 1:5,000
IHC: 1:200
IF：1：100-1：200
Antibody can detects endogenous β-tubulin protein.
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist
principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are
useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in
certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used
as loading control for these tissues.
1.Xiaoyu D ,Yiting F ,Dongqin X , et al.Targeting macrophagic 17β-HSD7 by fenretinide for the treatment of nonalcoholic fatty liver disease[J].Acta Pharmaceutica Sinica B,2023,13(1):142-156. (IF 14.903)
2.Ding F ,Zheng P ,Fang T H , et al.Adipocyte-specific FAK deletion promotes pancreatic β-cell apoptosis via adipose inflammatory response to exacerbate diabetes mellitus.[J].Clinical and translational medicine,2024,14(7):e1742. (IF 7.9)
3.Ding F ,Zheng P ,Yan Y X , et al.Adipocyte-secreted PRELP promotes adipocyte differentiation and adipose tissue fibrosis by binding with p75NTR to activate FAK/MAPK signaling.[J].International journal of biological macromolecules,2024,279(P4):135376. (IF 7.7)
4.Chen H ,Liu P ,Yu R , et al.Swertiamarin ameliorates type 2 diabetes by activating ADRB3/UCP1 thermogenic signals in adipose tissue[J].Phytomedicine,2024,135156190-156190. (IF 6.7)
5.HuiJian C ,XiYue Y ,Ao S , et al.High-Fat-Diet-Induced Extracellular Matrix Deposition Regulates Integrin - FAK Signals in Adipose Tissue to Promote Obesity.[J].Molecular nutrition & food research,2022,66(7):e2101088-e2101088. (IF 6.575)
6.Wushuang H ,Xueqing Z ,Mei Y , et al.PER2-mediated ameloblast differentiation via PPARγ/AKT1/β-catenin axis.[J].International journal of oral science,2021,13(1):16-16. (IF 6.344)
7.HuiJian C ,XiYue Y ,Ao S , et al.Adipose extracellular matrix deposition is an indicator of obesity and metabolic disorders.[J].The Journal of nutritional biochemistry,2022,111109159-109159. (IF 6.117)
8.Ning H ,LiuGen L ,XingChun P , et al.Ferroptosis triggered by dihydroartemisinin facilitates chlorin e6 induced photodynamic therapy by inhibiting GPX4 and enhancing ROS.[J].European journal of pharmacology,2022,919(prepublish):174797-174797. (IF 5.195)
P H ,S F W ,H X W , et al.RNA-sequencing analysis reveals potential molecular mechanism of RAD54B in the proliferation of inflamed human dental pulp cells.[J].International endodontic journal,2022,56(1):39-52. (IF 5.165)
答：可能的原因有：
1、目的蛋白有多个修饰位点，本身可以呈现多条带，建议查阅文献或进行生物信息学分析，获得蛋白序列的修饰位点信息，通过去修饰确定蛋白实际大小；
2、样本处理过程中目的蛋白发生降解,建议加入蛋白酶抑制剂；样本处理时在冰上操作；
3、杂蛋白多，建议处理目的蛋白；
4、抗体特异性不强，建议使用特异性强的抗体；
5、抗体孵育时间过久，建议减少抗体孵育时间；
6、二抗与抗原有交叉反应，建议选择合适的封闭物；
7、二聚体或多聚体存在，建议增加蛋白质变性过程及强度；
8、底物显色与曝光时间过长，建议缩短显色及曝光的时间。
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