β-tubulin Mouse Monoclonal Antibody(5G3)
货号:PMK181M/S
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价格:
580.00
规格:
50ul
100ul
货期:
现货
本产品4℃运输,保存于4℃,保质期 12个月。
Details
产品详情
  • Applications

    WB, IHC,IF

  • Species Cross-Reactivity

    H, R, M, Mk, Dg, C, Hm, Rb, Sh

  • Molecular Weight

    55KD

  • Isotype

    IgG1

  • Storage Buffer & Condition

    PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.Store at -20°C. 

    Do not aliquot the antibody.


  • Recommended dilutions

    WB: 1:5,000   

    IHC: 1:200    

    IF:1:100-1:200


  • Specificity

    Antibody can detects endogenous β-tubulin protein.

  • Background

    Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist 

    principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are 

    useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in 

    certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used 

    as loading control for these tissues.


Instruction manual/COA download
说明书/COA下载
Published literature
已发表文献
  • 更新中...

Product Q&A FAQ
产品问答FAQ
  • Western Blot(WB)实验中有很多杂带咋回事?

    答:可能的原因有:

    1、目的蛋白有多个修饰位点,本身可以呈现多条带,建议查阅文献或进行生物信息学分析,获得蛋白序列的修饰位点信息,通过去修饰确定蛋白实际大小;

    2、样本处理过程中目的蛋白发生降解,建议加入蛋白酶抑制剂;样本处理时在冰上操作;

    3、杂蛋白多,建议处理目的蛋白;

    4、抗体特异性不强,建议使用特异性强的抗体;

    5、抗体孵育时间过久,建议减少抗体孵育时间;

    6、二抗与抗原有交叉反应,建议选择合适的封闭物;

    7、二聚体或多聚体存在,建议增加蛋白质变性过程及强度;

    8、底物显色与曝光时间过长,建议缩短显色及曝光的时间。


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