Strep Tag II Mouse Monoclonal Antibody
货号:PMK562M/PMK562S
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价格:
580.00
规格:
50ul
100ul
货期:
现货
本产品4℃运输,保存于-20℃,保质期 12个月。
Details
产品详情
  • Catalog:

    PMK562M               PMK562S

  • Quantity:

      50ul                       100ul

  • Applications:

    WB, IHC

  • Species Cross-Reactivity:

    Recombinant Strep Tag II Specific

  • Molecular Weight:

    Strep Tag II

  • Isotype:

    IgG1

  • Storage Buffer & Condition:

    PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.Store at -20°C. 

    Do not aliquot the antibody.


  • Recommended dilutions:

    WB: 1:5,000   IHC: 1:200 

  • Specificity:

    The Strep Tag II Mouse Monoclonal Antibody can detects Strep Tag II recombinant protein.

  • Background:

    Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide used in numerous molecular biological 

    protocols dues to its strong affinity for biotin. NWSHPQFEK (Strep tag II) is a nine amino acid peptide with high specificity 

    and affinity towards streptavidin. NWSHPQFEK Tag Antibody is a useful tool in analysis and affinity purification of Strep tag 

    II fusion proteins.


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  • Application:

    WB-Western blot

    IHC-Immunochemistry

    IF-Immunofluorescence

    IP-Immunoprecipitation

    ChIP-Chormatin Immunoprecipitation


  • Reactivity:

    H-Human      R-Rat               M-Mouse       Mk-Monkey    Dg-Dog

    Ch-Chicken   Hm-Hamster   Rb-Rabbit       Sh-Sheep        Pg-Pig


Published literature
已发表文献
  • 更新中...

Product Q&A FAQ
产品问答FAQ
  • Western Blot(WB)实验中有很多杂带咋回事?

    答:可能的原因有:

    1、目的蛋白有多个修饰位点,本身可以呈现多条带,建议查阅文献或进行生物信息学分析,获得蛋白序列的修饰位点信息,通过去修饰确定蛋白实际大小;

    2、样本处理过程中目的蛋白发生降解,建议加入蛋白酶抑制剂;样本处理时在冰上操作;

    3、杂蛋白多,建议处理目的蛋白;

    4、抗体特异性不强,建议使用特异性强的抗体;

    5、抗体孵育时间过久,建议减少抗体孵育时间;

    6、二抗与抗原有交叉反应,建议选择合适的封闭物;

    7、二聚体或多聚体存在,建议增加蛋白质变性过程及强度;

    8、底物显色与曝光时间过长,建议缩短显色及曝光的时间。


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