Goat anti-human IgG (H+L), HRP Conjugated
货号:PMK-014-098M/S
一键复制产品信息
价格:
160.00
规格:
100ul
1ml
货期:
现货
本产品4℃运输,保存于4℃,保质期 12个月。
Details
产品详情
  • Applications

    WB, IHC

  • Species Cross-Reactivity

    Human

  • Molecular Weight

    N/A

  • Isotype

    IgG1

    Storage Buffer & Condition: PBS, pH 7.4, 15mg/ml Bovine Serum Albumin as Stablizer and  50% Glycerol. Store at -20°C. 

    Do not aliquot the antibody.


  • Suggested Dilution Range

    1:500-1:5,000 for immunohisto/cytochemistry

    1:3,000-1:100,00for ELLSA and Western blotting with chromogenic substrates 

    1:5,000-1:100,00 for Western blotting with ECL substates


  • Purity

    The antibody reacts with whole molecule human IgG. It also reacts with the light chains of other human immunoglobulins.

    Antibody Specificity:Based on immunoelectrophoresis and/or EL1SA,the antibody reacts with whole molecule mouse IgG. 

    lt also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin 

    serum proteins.The antibody may cross-react with immunoglobulins from other species.


  • Expiration date

    Store at -20°C. Avoid repeated freeze / thaw cycles.The expiration date may be extended if test results are acceptable for the 

    intended use.


  • Note

    For in vitro research use only,not for diagnostic or therapeutic use.This product is not a medical device.

Instruction manual/COA download
说明书/COA下载
Published literature
已发表文献
  • 1.H W ,M H ,R Y , et al.Astrocyte elevated gene-1 participates in the production of pro-inflammatory cytokines in dental pulp cells via NF-κB signalling pathway.[J].International endodontic journal,2018,51(10):1130-1138.(IF 3.015)

Product Q&A FAQ
产品问答FAQ
  • Western Blot(WB)实验中有很多杂带咋回事?

    答:可能的原因有:

    1、目的蛋白有多个修饰位点,本身可以呈现多条带,建议查阅文献或进行生物信息学分析,获得蛋白序列的修饰位点信息,通过去修饰确定蛋白实际大小;

    2、样本处理过程中目的蛋白发生降解,建议加入蛋白酶抑制剂;样本处理时在冰上操作;

    3、杂蛋白多,建议处理目的蛋白;

    4、抗体特异性不强,建议使用特异性强的抗体;

    5、抗体孵育时间过久,建议减少抗体孵育时间;

    6、二抗与抗原有交叉反应,建议选择合适的封闭物;

    7、二聚体或多聚体存在,建议增加蛋白质变性过程及强度;

    8、底物显色与曝光时间过长,建议缩短显色及曝光的时间。


Copyright ©湖北普美生物科技有限公司 All Rights Reserved.    工信部备案:鄂ICP备17001029号-1    技术支持:武汉微盟

  • 首页
  • 电话
  • 咨询
  • 顶部