WB, IHC
Mouse
N/A
IgG1
PBS, pH 7.4, 15mg/ml Bovine Serum Albumin as Stablizer and 50% Glycerol. Store at -20°C.
Do not aliquot the antibody.
1:500-1:5,000 for immunohisto/cytochemistry
1:5,000-1:100,000 for ELLSA and Western blotting with chromogenic substrates
1:10,000-1:200,000 for Western blotting with ECL substates
The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Based on immunoelectrophoresis and/or EL1SA,the antibody reacts with whole molecule mouse IgG. lt also reacts with the
light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.The
antibody may cross-react with immunoglobulins from other species.
Store at -20°C. Avoid repeated freeze / thaw cycles.The expiration date may be extended if test results are acceptable for the
intended use.
For in vitro research use only,not for diagnostic or therapeutic use.This product is not a medical device.
1. Ying T ,Yongyong Y ,Yue W , et al.Developmental exposure to chlorpyrifos causes neuroinflammation via necroptosis in mouse hippocampus and human microglial cell line.[J].Environmental pollution (Barking, Essex : 1987),2022,314120217-120217. (IF 9.988)
2.Jun Y ,Yingcheng Z ,Peipei Y , et al.Novel Host Protein TBC1D16, a GTPase Activating Protein of Rab5C, Inhibits Prototype Foamy Virus Replication.[J].Frontiers in immunology,2021,12658660-658660. (IF 7.561)
3. Lulu D ,Shasha C ,Jiang L , et al.Synthesis and biological evaluation of novel schisanhenol derivatives as potential hepatoprotective agents.[J].European journal of medicinal chemistry,2021,227113919-113919. (IF 6.514)
4. Xin W ,MingJing H ,XiaoJie C , et al.Glaucocalyxin A impairs tumor growth via amplification of the ATF4/CHOP/CHAC1 cascade in human oral squamous cell carcinoma.[J].Journal of ethnopharmacology,2022,290115100-115100. (IF 5.195)
5.Yongxi D ,Jun L ,Shanhui Z , et al.Design, synthesis and bioevaluation of 1,2,4-thiadiazolidine-3,5-dione derivatives as potential GSK-3β inhibitors for the treatment of Alzheimer's disease[J].Bioorganic Chemistry,2023,134106446-106446. (IF 5.1)
6. Xueqing Z ,Wushuang H ,Zhenru H , et al.Effects of Fam83h truncation mutation on enamel developmental defects in male C57/BL6J mice.[J].Bone,2022,166116595-116595. (IF 4.626)
7. Sun Y ,Chen Y ,Zhao H , et al.Lactate-driven type I collagen deposition facilitates cancer stem cell-like phenotype of head and neck squamous cell carcinoma[J].iScience,2024,27(4):109340-. (IF 4.6)
8. Chengcan Y ,Qian G ,Nuo X , et al.Human dental pulp stem cells are subjected to metabolic reprogramming and repressed proliferation and migration by the sympathetic nervous system via ADRA1B.[J].Journal of endodontics,2023,49(12):1641-1651.e6. (IF 4.2)
9. Peipei Y ,Lanlan D ,Qingqing C , et al.Prototype foamy virus elicits complete autophagy involving the ER stress-related UPR pathway.[J].Retrovirology,2017,14(1):16. (IF 3.867)
10. Wang Y ,Mao Y ,Zhang X , et al.TAK1 may promote the development of diabetic nephropathy by reducing the stability of SnoN protein[J].Life Sciences,2019,2281-10. (IF 3.448)
答：可能的原因有：
1、目的蛋白有多个修饰位点，本身可以呈现多条带，建议查阅文献或进行生物信息学分析，获得蛋白序列的修饰位点信息，通过去修饰确定蛋白实际大小；
2、样本处理过程中目的蛋白发生降解,建议加入蛋白酶抑制剂；样本处理时在冰上操作；
3、杂蛋白多，建议处理目的蛋白；
4、抗体特异性不强，建议使用特异性强的抗体；
5、抗体孵育时间过久，建议减少抗体孵育时间；
6、二抗与抗原有交叉反应，建议选择合适的封闭物；
7、二聚体或多聚体存在，建议增加蛋白质变性过程及强度；
8、底物显色与曝光时间过长，建议缩短显色及曝光的时间。
Copyright ©湖北普美生物科技有限公司 All Rights Reserved. 工信部备案：鄂ICP备17001029号-1 技术支持：武汉微盟
