PBS, pH 7.4, 15mg/ml Bovine Serum Albumin as Stablizer and 50% Glycerol.Store at -20°C. Do not aliquot the antibody.
1:500-1:5,000 for immunohisto/cytochemistry
1:5,000-1:100,000 for ELISA and Western blotting with chromogenic
substrates 1:10,000-1:200,000 for Western blotting with ECL substates
The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled
to agarose beads.
Based on immunoelectrophoresis and/or ELISA,the antibody reacts with whole molecule rabbit IgG. lt also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
The antibody may cross-react with immunoglobulins from other species.
Store at -20°C. Avoid repeated freeze / thaw cycles.The expiration date may be extended if test results are acceptable for the intended use.
For in vitro research use only,not for diagnostic or therapeutic use.This product is not a medical device.
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答：可能的原因有：
1、目的蛋白有多个修饰位点，本身可以呈现多条带，建议查阅文献或进行生物信息学分析，获得蛋白序列的修饰位点信息，通过去修饰确定蛋白实际大小；
2、样本处理过程中目的蛋白发生降解,建议加入蛋白酶抑制剂；样本处理时在冰上操作；
3、杂蛋白多，建议处理目的蛋白；
4、抗体特异性不强，建议使用特异性强的抗体；
5、抗体孵育时间过久，建议减少抗体孵育时间；
6、二抗与抗原有交叉反应，建议选择合适的封闭物；
7、二聚体或多聚体存在，建议增加蛋白质变性过程及强度；
8、底物显色与曝光时间过长，建议缩短显色及曝光的时间。
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