Flow Cyt,ICC/IF
Chicken
150KD
IgG
0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6.Store at -20°C.
Do not aliquot the antibody.
2mg/ml
Goat Polyclonal IgG, affinity purified by Protein A.
Flow-Cyt=1:100-1000 IF=1:100-1000
Fluorescein-5-isothiocyanate(FITC)Amax=495nm;Emax=519nm
The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult
blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a
part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited
the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by
somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Store at -20°C. Avoid repeated freeze / thaw cycles.The expiration date may be extended if test results are acceptable for
the intended use.
For in vitro research use only,not for diagnostic or therapeutic use.This product is not a medical device.
1.Zhou Y ,Dong W ,Wang L , et al.Cystatin C Attenuates Perihematomal Secondary Brain Injury by Inhibiting the Cathepsin B/NLRP3 Signaling Pathway in a Rat Model of Intracerebral Hemorrhage.[J].Molecular neurobiology,2024,61(11):9646-9662. (IF 4.6)
2.HuiJian C ,XiYue Y ,Ao S , et al.High-Fat-Diet-Induced Extracellular Matrix Deposition Regulates Integrin - FAK Signals in Adipose Tissue to Promote Obesity.[J].Molecular nutrition & food research,2022,66(7):e2101088-e2101088. (IF 6.757)
3.Zhao Y ,Wang P ,Sun X , et al.Candida albicansInfection Disrupts the Metabolism of Vaginal Epithelial Cells and Inhibits Cellular Glycolysis[J].Microorganisms,2024,12(2): (IF 4.1)
4.Sun Y ,Chen Y ,Zhao H , et al.Lactate-driven type I collagen deposition facilitates cancer stem cell-like phenotype of head and neck squamous cell carcinoma[J].iScience,2024,27(4):109340-. (IF 4.6)
答:可能的原因有:
1、目的蛋白有多个修饰位点,本身可以呈现多条带,建议查阅文献或进行生物信息学分析,获得蛋白序列的修饰位点信息,通过去修饰确定蛋白实际大小;
2、样本处理过程中目的蛋白发生降解,建议加入蛋白酶抑制剂;样本处理时在冰上操作;
3、杂蛋白多,建议处理目的蛋白;
4、抗体特异性不强,建议使用特异性强的抗体;
5、抗体孵育时间过久,建议减少抗体孵育时间;
6、二抗与抗原有交叉反应,建议选择合适的封闭物;
7、二聚体或多聚体存在,建议增加蛋白质变性过程及强度;
8、底物显色与曝光时间过长,建议缩短显色及曝光的时间。
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