Flow Cyt,ICC/IF,IHC
Mouse
N/A
IgG1
0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6 Store at -20°C.
Do not aliquot the antibody.
15mg/ml Bovine Serum Albumin (IgG-Free,Protease-Free)
0.05% Sodium Azide
1:100-1:800 for most applications
Fluorescein-5-isothiocyanate(FITC)Amax=492nm;Emax=520nm
The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to
agarose beads.
Based on immunoelectrophoresis and/or EL1SA,the antibody reacts with whole molecule mouse IgG. lt also
reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-
immunoglobulin serum proteins.The antibody may cross-react with immunoglobulins from other species.
Store at -20°C. Avoid repeated freeze / thaw cycles.The expiration date may be extended if test results are
acceptable for the intended use.
For in vitro research use only,not for diagnostic or therapeutic use.This product is not a medical device.
1.Tingting L ,Xiazhou F ,Yaoli L , et al.Sulforaphane Inhibits Osteoclastogenesis via Suppression of the Autophagic Pathway[J].Molecules,2021,26(2):347-347. (IF 4.412)
2.Sun Y ,Chen Y ,Zhao H , et al.Lactate-driven type I collagen deposition facilitates cancer stem cell-like phenotype of head and neck squamous cell carcinoma[J].iScience,2024,27(4):109340-. (IF 4.6)
3.HuiJian C ,XiYue Y ,Ao S , et al.High-Fat-Diet-Induced Extracellular Matrix Deposition Regulates Integrin - FAK Signals in Adipose Tissue to Promote Obesity.[J].Molecular nutrition & food research,2022,66(7):e2101088-e2101088. (IF 6.575)
4.Wangming Z ,Xiaojing Y ,Linchun B , et al.Bisbenzylisoquinoline alkaloid fangchinoline derivative HY-2 inhibits breast cancer cells by suppressing BLM DNA helicase.[J].Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie,2023,169115908-115908. (IF 7.5)
5.Yongxi D ,Jun L ,Shanhui Z , et al.Design, synthesis and bioevaluation of 1,2,4-thiadiazolidine-3,5-dione derivatives as potential GSK-3β inhibitors for the treatment of Alzheimer's disease[J].Bioorganic Chemistry,2023,134106446-106446. (IF 5.1)
答：可能的原因有：
1、目的蛋白有多个修饰位点，本身可以呈现多条带，建议查阅文献或进行生物信息学分析，获得蛋白序列的修饰位点信息，通过去修饰确定蛋白实际大小；
2、样本处理过程中目的蛋白发生降解,建议加入蛋白酶抑制剂；样本处理时在冰上操作；
3、杂蛋白多，建议处理目的蛋白；
4、抗体特异性不强，建议使用特异性强的抗体；
5、抗体孵育时间过久，建议减少抗体孵育时间；
6、二抗与抗原有交叉反应，建议选择合适的封闭物；
7、二聚体或多聚体存在，建议增加蛋白质变性过程及强度；
8、底物显色与曝光时间过长，建议缩短显色及曝光的时间。
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