Goat anti-Rat IgG (H+L), FITC AffiniPure
货号:PMK-014-100M/S
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价格:
160.00
规格:
100ul
1ml
货期:
现货
本产品4℃运输,保存于4℃,保质期 12个月。
Details
产品详情
  • For research use only
    ApplicationsSpecies Cross-ReactivityMolecular WeightIsotype
    Flow Cyt,ICC/IF,IHC
    Rat
    N/AIgG1
  • Storage Buffer & Condition

    0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6 Store at -20°C.Do not aliquot the antibody.

  • Stabilizer

    15mg/ml Bovine Serum Albumin (IgG-Free,Protease-Free)

  • Preservative

    0.05% Sodium Azide

  • Suggested Dilution Range

    1:100-1:800 for most applications

  • Fluorophore

    Fluorescein-5-isothiocyanate(FITC)
    Amax=492nm;Emax=520nm

  • Purity

    The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

  • Antibody Specificity

    Based on immunoelectrophoresis and/or EL1SA,the antibody reacts with whole molecule Rat IgG. lt also reacts with the light chains of other Rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
    The antibody may cross-react with immunoglobulins from other species.

  • Expiration date

    Store at -20°C. Avoid repeated freeze / thaw cycles.The expiration date may be extended if test results are acceptable for the intended use.

  • Note

    For in vitro research use only,not for diagnostic or therapeutic use.This product is not a medical device.

Instruction manual/COA download
说明书/COA下载
Published literature
已发表文献
  • 更新中...

Product Q&A FAQ
产品问答FAQ
  • Western Blot(WB)实验中有很多杂带咋回事?

    答:可能的原因有:

    1、目的蛋白有多个修饰位点,本身可以呈现多条带,建议查阅文献或进行生物信息学分析,获得蛋白序列的修饰位点信息,通过去修饰确定蛋白实际大小;

    2、样本处理过程中目的蛋白发生降解,建议加入蛋白酶抑制剂;样本处理时在冰上操作;

    3、杂蛋白多,建议处理目的蛋白;

    4、抗体特异性不强,建议使用特异性强的抗体;

    5、抗体孵育时间过久,建议减少抗体孵育时间;

    6、二抗与抗原有交叉反应,建议选择合适的封闭物;

    7、二聚体或多聚体存在,建议增加蛋白质变性过程及强度;

    8、底物显色与曝光时间过长,建议缩短显色及曝光的时间。


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