Flow Cyt ,ICC/IF，IHC
Mouse
N/A
IgG1
0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6 Store at -20°C.
Do not aliquot the antibody.
15mg/ml Bovine Serum Albumin (IgG-Free,Protease-Free)
0.05% Sodium Azide
1:100-1:800 for most applications
Cy3 bis-NHS esterAmax=550 nm; Emax=570 nm
The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to
agarose beads.
Based on immunoelectrophoresis and/or EL1SA,the antibody reacts with whole molecule mouse IgG. lt also
reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-
immunoglobulin serum proteins.The antibody may cross-react with immunoglobulins from other species.
Store at -20°C. Avoid repeated freeze / thaw cycles.The expiration date may be extended if test results are
acceptable for the intended use.
For in vitro research use only,not for diagnostic or therapeutic use.This product is not a medical device.
1. Rongfeng Yang, Chunlei Wen, Zijun Wang, Sevoflurane preconditioning improves Cx43 localization and electrical conduction by stabilizing myocardial microtubule structure during ischemia-reperfusion. (IF 2.2)
2.Huimin Sun, Jiahui Zou, Genome-wide CRISPR screen identifies STK11 as a critical regulator of sialic acid clusters important for influenza A virus attachment,Journal of Advanced Research,2025.(IF 13)
3.Chunlei Wen, Rongfeng Yang,Downregulation of EB1 impedes Cx43 localization and cardiac conduction after hypothermic ischemia-reperfusion in rats,PeerJ,2025.(IF 2.4)
4.Zixin Zhu, Ya Zhang,Thymosin beta 4 alleviates non-alcoholic fatty liver by inhibiting ferroptosis via up-regulation of GPX4,EUROPEAN JOURNAL OF PHARMACOLOGY,2021(IF 4.432)
5.Yunqing Sun, Yang Chen,Lactate-driven type I collagen deposition facilitates cancer stem cell-like phenotype of head and neck squamous cell carcinoma,iScience,2024.（IF 4.6）
6.1.Yongxi Dong, Jun Lu,Integration of Network Pharmacology, Transcriptomics, and .Strategies to Uncover the Mechanism of Chaihuang Qingfu Pill in Treating Sepsis-Induced Liver Injury,Drug Design Development and Therapy,2025. (IF 5.1)
答：可能的原因有：
1、目的蛋白有多个修饰位点，本身可以呈现多条带，建议查阅文献或进行生物信息学分析，获得蛋白序列的修饰位点信息，通过去修饰确定蛋白实际大小；
2、样本处理过程中目的蛋白发生降解,建议加入蛋白酶抑制剂；样本处理时在冰上操作；
3、杂蛋白多，建议处理目的蛋白；
4、抗体特异性不强，建议使用特异性强的抗体；
5、抗体孵育时间过久，建议减少抗体孵育时间；
6、二抗与抗原有交叉反应，建议选择合适的封闭物；
7、二聚体或多聚体存在，建议增加蛋白质变性过程及强度；
8、底物显色与曝光时间过长，建议缩短显色及曝光的时间。
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