Flow Cyt,ICC/IF,IHC
Rabbit
N/A
IgG1
0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6 Store at -20°C.
Do not aliquot the antibody.
15mg/ml Bovine Serum Albumin (IgG-Free,Protease-Free)
0.05% Sodium Azide
1:100-1:800 for most applications
Cy3 bis-NHS esterAmax=550 nm; Emax=570 nm
The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to
agarose beads.
Based on immunoelectrophoresis and/or EL1SA,the antibody reacts with whole molecule rabbit IgG. lt also
reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-
immunoglobulin serum proteins.The antibody may cross-react with immunoglobulins from other species.
Store at -20°C. Avoid repeated freeze / thaw cycles.The expiration date may be extended if test results are
acceptable for the intended use.
For in vitro research use only,not for diagnostic or therapeutic use.This product is not a medical device.
更新中...
答:可能的原因有:
1、目的蛋白有多个修饰位点,本身可以呈现多条带,建议查阅文献或进行生物信息学分析,获得蛋白序列的修饰位点信息,通过去修饰确定蛋白实际大小;
2、样本处理过程中目的蛋白发生降解,建议加入蛋白酶抑制剂;样本处理时在冰上操作;
3、杂蛋白多,建议处理目的蛋白;
4、抗体特异性不强,建议使用特异性强的抗体;
5、抗体孵育时间过久,建议减少抗体孵育时间;
6、二抗与抗原有交叉反应,建议选择合适的封闭物;
7、二聚体或多聚体存在,建议增加蛋白质变性过程及强度;
8、底物显色与曝光时间过长,建议缩短显色及曝光的时间。
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