Goat anti-Rat IgG (H+L), PE AffiniPure
货号:PMK-015-099M/S
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价格:
240.00
规格:
100ul
1ml
货期:
现货
本产品4℃运输,保存于4℃,保质期 12个月。
Details
产品详情
  • Applications

    IF、Flow-Cyt

  • Species Cross-Reactivity

    Rat

  • Molecular Weight

    N/A

  • Isotype

    IgG1

  • Storage Buffer & Condition

    0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6 Store at -20°C.Do not aliquot the antibody.

  • Concentration

    2mg/ml

  • Preservative

    0.05% Sodium Azide

  • Also known as

    Immunoglobulin G

  • Application

    Flow-Cyt: 1:100-1000     IF:  1:100-1000 

    not yet tested in other applications.


  • Purity

    The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

  • background

    mmunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. 

    IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our 

    adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response 

    and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic 

    recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.


  • Expiration date

    Store at -20°C. Avoid repeated freeze / thaw cycles.The expiration date may be extended if test results are acceptable 

    for the intended use.


  • Note

    For in vitro research use only,not for diagnostic or therapeutic use.This product is not a medical device.

Instruction manual/COA download
说明书/COA下载
Published literature
已发表文献
  • 更新中...

Product Q&A FAQ
产品问答FAQ
  • Western Blot(WB)实验中有很多杂带咋回事?

    答:可能的原因有:

    1、目的蛋白有多个修饰位点,本身可以呈现多条带,建议查阅文献或进行生物信息学分析,获得蛋白序列的修饰位点信息,通过去修饰确定蛋白实际大小;

    2、样本处理过程中目的蛋白发生降解,建议加入蛋白酶抑制剂;样本处理时在冰上操作;

    3、杂蛋白多,建议处理目的蛋白;

    4、抗体特异性不强,建议使用特异性强的抗体;

    5、抗体孵育时间过久,建议减少抗体孵育时间;

    6、二抗与抗原有交叉反应,建议选择合适的封闭物;

    7、二聚体或多聚体存在,建议增加蛋白质变性过程及强度;

    8、底物显色与曝光时间过长,建议缩短显色及曝光的时间。


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