WB、IHC、ELISA
Chicken
N/A
IgY
PBS, pH 7.4, 15mg/ml Bovine Serum Albumin as Stablizer and 50% Glycerol. Store at -20°C.
Do not aliquot the antibody.
1:100-1:1,000 for immunohisto/cytochemistry
1:1,000-1:100,00 for ELLSA and Western blotting with chromogenic substrates
1:1,000-1:10,000 for Western blotting with ECL substates Optimal dilutions should be determined by the end user.
The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Antibody Specificity:Based on immunoelectrophoresis and/or EL1SA,the antibody reacts with whole molecule pig IgG.
lt also reacts with the light chains of other pig immunoglobulins. No antibody was detected against non-immunoglobulin
serum proteins.The antibody may cross-react with immunoglobulins from other species.
Store at -20°C. Avoid repeated freeze / thaw cycles.The expiration date may be extended if test results are acceptable for
the intended use.
For in vitro research use only,not for diagnostic or therapeutic use.This product is not a medical device.
更新中...
答:可能的原因有:
1、目的蛋白有多个修饰位点,本身可以呈现多条带,建议查阅文献或进行生物信息学分析,获得蛋白序列的修饰位点信息,通过去修饰确定蛋白实际大小;
2、样本处理过程中目的蛋白发生降解,建议加入蛋白酶抑制剂;样本处理时在冰上操作;
3、杂蛋白多,建议处理目的蛋白;
4、抗体特异性不强,建议使用特异性强的抗体;
5、抗体孵育时间过久,建议减少抗体孵育时间;
6、二抗与抗原有交叉反应,建议选择合适的封闭物;
7、二聚体或多聚体存在,建议增加蛋白质变性过程及强度;
8、底物显色与曝光时间过长,建议缩短显色及曝光的时间。
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