WB

N/A

N/A

IgG

PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C. 

Do not aliquot the antibody.

WB: 1:5,000

The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion proteins.

The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with thebioactivity 

or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expressionvectors. 

It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type 

proteinex pressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A 

Flag-tag can beused in many different assays that require recognition by an antibody. If there is no antibody against the 

studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence