WB, IHC
H, R, M, Mk, Ch, Hm, Sh，Pg，Xenopus
37KD
IgG1
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.Store at -20°C.
Do not aliquot the antibody.
WB: 1:5,000
IHC: 1:200
Antibody can detects endogenous GAPDH protein.
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis.
GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as
loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH
expression in certain cell types.
1.Xiao-Long W ,Shuo W ,Zhi-Zhong W , et al.Overexpression of ATAD2 indicates Poor Prognosis in Oral Squamous Cell Carcinoma.[J].International journal of medical sciences,2020,17(11):1598-1609.(IF 2.523)
2.Bing H ,Yi Y ,Lei T , et al.Roles of SET7/9 and LSD1 in the Pathogenesis of Arsenic-induced Hepatocyte Apoptosis.[J].Journal of clinical and translational hepatology,2021,9(3):364-372.(IF 4.108)
3.Liyuan H ,Fangfang S ,Hualing S , et al.IRX5 promotes NF-κB signalling to increase proliferation, migration and invasion via OPN in tongue squamous cell carcinoma.[J].Journal of cellular and molecular medicine,2018,22(8):3899-3910.(IF 4.302)
4.Xueqing Z ,Wushuang H ,Zhenru H , et al.Effects of Fam83h truncation mutation on enamel developmental defects in male C57/BL6J mice.[J].Bone,2022,166116595-116595.(IF 4.626)
5.De-Run C ,Yuan G ,Yao X , et al.Engineered nanogels simultaneously implement HDAC inhibition and chemotherapy to boost antitumor immunity via pyroptosis[J].Applied Materials Today,2022,26(IF 8.663)
6.He F ,Wang F ,Xiang H , et al.Activation of adenosine A2B receptor alleviates myocardial ischemia-reperfusion injury by inhibiting endoplasmic reticulum stress and restoring autophagy flux.[J].Archives of biochemistry and biophysics,2024,754109945-109945.(IF 3.8)
答：可能的原因有：
1、目的蛋白有多个修饰位点，本身可以呈现多条带，建议查阅文献或进行生物信息学分析，获得蛋白序列的修饰位点信息，通过去修饰确定蛋白实际大小；
2、样本处理过程中目的蛋白发生降解,建议加入蛋白酶抑制剂；样本处理时在冰上操作；
3、杂蛋白多，建议处理目的蛋白；
4、抗体特异性不强，建议使用特异性强的抗体；
5、抗体孵育时间过久，建议减少抗体孵育时间；
6、二抗与抗原有交叉反应，建议选择合适的封闭物；
7、二聚体或多聚体存在，建议增加蛋白质变性过程及强度；
8、底物显色与曝光时间过长，建议缩短显色及曝光的时间。
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