Lamin B1 Rabbit Polyclonal Antibody
货号:PMK050M/S
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价格:
580.00
规格:
50ul
100ul
货期:
现货
本产品4℃运输,保存于4℃,保质期 12个月。
Details
产品详情
  • Applications

    WB, IP

  • Species Cross-Reactivity

    H, R, M

  • Molecular Weight

    68KD

  • Isotype

    IgG1

  • Storage Buffer & Condition

    PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20 °C.

    Do not aliquot the antibody.


  • Recommended dilutions

    WB: 1:1,0 00-3,000 

    IHC: 1:500-1,000


  • Specificity

    The Lamin B1 antibody ca n detects endogenous Lamin B1 protein.

  • Alternative Names

    ADLD, LMB1,LMNB1 , MGC111419, OTTHUMP00000159218

  • Background

    The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. 

    The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina 

    matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be 

    involved in nuclear stability, chromatin structure and gene expression. Vertebrate la mins consist of two types, A 

    and B. This gene encodes one of the two B type proteins, B1.


Instruction manual/COA download
说明书/COA下载
Published literature
已发表文献
  • 更新中...

Product Q&A FAQ
产品问答FAQ
  • Western Blot(WB)实验中有很多杂带咋回事?

    答:可能的原因有:

    1、目的蛋白有多个修饰位点,本身可以呈现多条带,建议查阅文献或进行生物信息学分析,获得蛋白序列的修饰位点信息,通过去修饰确定蛋白实际大小;

    2、样本处理过程中目的蛋白发生降解,建议加入蛋白酶抑制剂;样本处理时在冰上操作;

    3、杂蛋白多,建议处理目的蛋白;

    4、抗体特异性不强,建议使用特异性强的抗体;

    5、抗体孵育时间过久,建议减少抗体孵育时间;

    6、二抗与抗原有交叉反应,建议选择合适的封闭物;

    7、二聚体或多聚体存在,建议增加蛋白质变性过程及强度;

    8、底物显色与曝光时间过长,建议缩短显色及曝光的时间。


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