WB, IP
H, R, M
68KD
IgG1
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20 °C.
Do not aliquot the antibody.
WB: 1:1,0 00-3,000
IHC: 1:500-1,000
The Lamin B1 antibody ca n detects endogenous Lamin B1 protein.
ADLD, LMB1,LMNB1 , MGC111419, OTTHUMP00000159218
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane.
The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina
matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be
involved in nuclear stability, chromatin structure and gene expression. Vertebrate la mins consist of two types, A
and B. This gene encodes one of the two B type proteins, B1.
更新中...
答:可能的原因有:
1、目的蛋白有多个修饰位点,本身可以呈现多条带,建议查阅文献或进行生物信息学分析,获得蛋白序列的修饰位点信息,通过去修饰确定蛋白实际大小;
2、样本处理过程中目的蛋白发生降解,建议加入蛋白酶抑制剂;样本处理时在冰上操作;
3、杂蛋白多,建议处理目的蛋白;
4、抗体特异性不强,建议使用特异性强的抗体;
5、抗体孵育时间过久,建议减少抗体孵育时间;
6、二抗与抗原有交叉反应,建议选择合适的封闭物;
7、二聚体或多聚体存在,建议增加蛋白质变性过程及强度;
8、底物显色与曝光时间过长,建议缩短显色及曝光的时间。
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