WB, IHC, IF

H, R, M, Mk, Ch, Hm, Sh,Xenopus

43KD

IgG1

PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C. 

Do not aliquot the antibody.

WB: 1:3,000   

IHC: 1:200     

IF: 1:200

Antibody can detects endogenous β-actin protein.

β-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and 

tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful 

as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. 

For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control 

for these tissues.