WB, IHC, IF
H, R, M, Mk, Ch, Hm, Sh,Xenopus
43KD
IgG1
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C.
Do not aliquot the antibody.
WB: 1:3,000
IHC: 1:200
IF: 1:200
Antibody can detects endogenous β-actin protein.
β-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and
tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful
as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells.
For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control
for these tissues.
1.MingJun S ,PingPing T ,ZhongQiang L , et al.MicroRNA-27a targets Sfrp1 to induce renal fibrosis in diabetic nephropathy by activating Wnt/β-Catenin signalling.[J].Bioscience reports,2020,40(6):(IF 2.942)
2.Liu L ,Wang Y ,Yan R , et al.BMP-7 inhibits renal fibrosis in diabetic nephropathy via miR-21 downregulation[J].Life Sciences,2019,238(C):116957.(IF 3.448)
3.Liyuan H ,Hualing S ,Fangfang S , et al.SIRT6 overexpression inhibits cementogenesis by suppressing glucose transporter 1.[J].Journal of cellular physiology,2019,234(4):4005-4014.(IF 3.923)
4.Xin S ,Shengyu W ,Xian L , et al.Proteome analysis of Duck Tembusu virus (DTMUV)-infected BHK-21 cells.[J].Proteomics,2017,17(12):n/a-n/a.(IF 4.041)
5.Liyuan H ,Fangfang S ,Hualing S , et al.IRX5 promotes NF-κB signalling to increase proliferation, migration and invasion via OPN in tongue squamous cell carcinoma.[J].Journal of cellular and molecular medicine,2018,22(8):3899-3910.(IF 4.302)
6.Yuanyuan W ,Lingling L ,Wei P , et al.Ski-related novel protein suppresses the development of diabetic nephropathy by modulating transforming growth factor-β signaling and microRNA-21 expression.[J].Journal of cellular physiology,2019,234(10):17925-17936.(IF 4.522)
7.Wenqi L ,Shiwen K ,Huijian C , et al.A composition of ursolic acid derivatives from Ludwigia hyssopifolia induces apoptosis in throat cancer cells via the Akt/mTOR and mitochondrial signaling pathways and by modulating endoplasmic reticulum stress.[J].Journal of ethnopharmacology,2023,319(P3):117351-117351.(IF 5.4)
8.Huiming L ,Rui Y ,Luqun L , et al.The role of CDX2 in renal tubular lesions during diabetic kidney disease.[J].Aging,2021,13(5):(IF 5.682)
9.Jiayi X ,Huifang Z ,Xingcheng Z , et al.Atorvastatin Restores PPARα Inhibition of Lipid Metabolism Disorders by Downregulating miR-21 Expression to Improve Mitochondrial Function and Alleviate Diabetic Nephropathy Progression[J].Frontiers in Pharmacology,2022,13819787-819787.(IF 5.988)
10.Liyuan H ,Jie S ,Lingqing D , et al.Simultaneous acceleration of osteogenesis and angiogenesis by surface oxygen vacancies of rutile nanorods[J].Colloids and Surfaces B: Biointerfaces,2022,212(prepublish):112348-.(IF 5.999)
11.Yerlan B ,Wenqi L ,Muguli M , et al.Ferula ferulaeoides ethyl acetate extract induces apoptosis in esophageal cancer cells via mitochondrial and PI3K/Akt/Bad pathways[J].Arabian Journal of Chemistry,2023,16(12):(IF 6)
12.Qian Z ,Zhen H ,Huanyan Z , et al.Chromatin Accessibility Predetermines Odontoblast Terminal Differentiation.[J].Frontiers in cell and developmental biology,2021,9769193-769193.(IF 6.684)
13.HuiFang Z ,HuiMing L ,JiaYi X , et al.Alpha lipoamide inhibits diabetic kidney fibrosis via improving mitochondrial function and regulating RXRα expression and activation.[J].Acta pharmacologica Sinica,2022,44(5):1051-1065.(IF 7.169)
答：可能的原因有：
1、目的蛋白有多个修饰位点，本身可以呈现多条带，建议查阅文献或进行生物信息学分析，获得蛋白序列的修饰位点信息，通过去修饰确定蛋白实际大小；
2、样本处理过程中目的蛋白发生降解,建议加入蛋白酶抑制剂；样本处理时在冰上操作；
3、杂蛋白多，建议处理目的蛋白；
4、抗体特异性不强，建议使用特异性强的抗体；
5、抗体孵育时间过久，建议减少抗体孵育时间；
6、二抗与抗原有交叉反应，建议选择合适的封闭物；
7、二聚体或多聚体存在，建议增加蛋白质变性过程及强度；
8、底物显色与曝光时间过长，建议缩短显色及曝光的时间。
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