Catalog：PMK042M PMK042S
Quantity： 50ul 100ul
Applications：WB, IHC, IF
Species Cross-Reactivity：H, R, M, Mk, Dg, Ch, Hm, Rb, Pg, Sh
Molecular Weight：37KD
Isotype：IgG1
Storage Buffer & Condition:
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.Store at -20°C.
Do not aliquot the antibody.
Recommended dilutions:
WB: 1:5,000
IF: 1:100-1:500
Specificity:
Antibody can detects endogenous GAPDH protein.
Background:
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis.
GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as
loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH
expression in certain cell types.
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Application:
WB-Western blot
IHC-Immunochemistry
IF-Immunofluorescence
IP-Immunoprecipitation
ChIP-Chormatin Immunoprecipitation
Reactivity:
H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog
Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
