Catalog：PMK082M PMK082S
Quantity： 50ul 100ul
Applications：WB, IHC, IF
Species Cross-Reactivity：H, R, M, Mk, Ch, Hm, Sh,Xenopus
Molecular Weight：43KD
Isotype：IgG1
Storage Buffer & Condition:
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C.
Do not aliquot the antibody.
Recommended dilutions:
WB: 1:3,000
IHC: 1:200
IF: 1:200
Specificity:
Antibody can detects endogenous β-actin protein.
Background:
β-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and
tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful
as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells.
For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control
for these tissues.
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Application:
WB-Western blot
IHC-Immunochemistry
IF-Immunofluorescence
IP-Immunoprecipitation
ChIP-Chormatin Immunoprecipitation
Reactivity:
H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog
Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
