Catalog：PMK181M PMK181S
Quantity： 50ul 100ul
Applications：WB, IHC，IF
Species Cross-Reactivity：H, R, M, Mk, Dg, C, Hm, Rb, Sh
Molecular Weight：55KD
Isotype：IgG1
Storage Buffer & Condition:
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.Store at -20°C.
Do not aliquot the antibody.
Recommended dilutions:
WB: 1:5,000
IHC: 1:200
IF：1：100-1：200
Specificity:
Antibody can detects endogenous β-tubulin protein.
Background:
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist
principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are
useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in
certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used
as loading control for these tissues.
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Application:
WB-Western blot
IHC-Immunochemistry
IF-Immunofluorescence
IP-Immunoprecipitation
ChIP-Chormatin Immunoprecipitation
Reactivity:
H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog
Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
