Catalog：PMK001M PMK001S PMK001L
Quantity： 50ul 100ul 1ml
Applications：WB, IP, IF
Species Cross-Reactivity：N/A
Molecular Weight：N/A
Isotype：IgG1
Storage Buffer & Condition:
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C.
Do not aliquot the antibody.
Recommended dilutions:
WB: 1:5,000
IP: 1:200
IF: 1:1000
Specificity:
The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion proteins.
Background:
The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity
or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors.
It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein
expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can
be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein,
adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.
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Application:
WB-Western blot
IHC-Immunochemistry
IF-Immunofluorescence
IP-Immunoprecipitation
ChIP-Chormatin Immunoprecipitation
Reactivity:
H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog
Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
