Catalog：PMK111M PMK111S
Quantity： 50ul 100ul
Applications：WB
Species Cross-Reactivity：N/A
Molecular Weight：N/A
Isotype：IgG
Storage Buffer & Condition:
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C.
Do not aliquot the antibody.
Recommended dilutions:
WB: 1:5,000
Specificity:
The His tag antibody can recognize C-terminal, internal, and N-terminal His-tag fusion proteins.
Background:
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues,
often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can
greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify,
detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.
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Application:
WB-Western blot
IHC-Immunochemistry
IF-Immunofluorescence
IP-Immunoprecipitation
ChIP-Chormatin Immunoprecipitation
Reactivity:
H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog
Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
