Catalog:PMK041M PMK041S
Quantity: 50ul 100ul
Applications:WB,IHC
Species Cross-Reactivity:H,R,M
Molecular Weight:29KD
Isotype:IgG1
Storage Buffer & Condition:
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Store at -20°C. Do not aliquot the antibody.
Recommended dilutions:
WB: 1:5,000 IHC: 1:200
Specificity:
Antibody can detects endogenous PCNA protein.
Background:
PCNA is a marker for cells in early G1 phase and Sphase of the cell cycle. It is found in the nucleus
and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the
processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA
is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants
encoding the same protein have been found for PCNA. Pseudogenes of this gene have been
described on chromosome 4 and on the X chromosome.
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Application:
WB-Western blot
IHC-Immunochemistry
IF-Immunofluorescence
IP-Immunoprecipitation
ChIP-Chormatin Immunoprecipitation
Reactivity:
H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog
Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig