Known As:Hepatocyte growth factor; HPTA; HGF; SF; Scatter factor; Hepatopoietin-A
PROPERTIES
Description:Recombinant Human Hepatocyte Growth Factor is produced by our Mammalian expression system and the target gene encoding Gln32-Ser728 is expressed with a 6His tag at the C-terminus.
Accession:P14210
Formulation:Lyophilized from a 0.2 μm filtered solution of 20mM Tris,500mM
NaCl,3%Trehalose,0.02% Tween 80,pH 8.0.
Size:10µg/50µg/500µg/1mg
Purity:> 95%
Endotoxin:< 0.01 EU/µg as determined by LAL test.
Predicted Mol Mass:26&53.7 KDa
Apparent Mol Mass:32-38&50-65 KDa, reducing conditions
Reconstitution:Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Shipping:The product is shipped at ambient temperature.
Upon receipt, store it immediately at the temperature listed below.
Storage:Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Background:HGF, is a pleiotropic protein in the Plasminogen subfamily of S1 peptidases and
contains 4 kringle domains, 1 PAN domain and 1 peptidase S1 domain. HGF is
secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide-linked HGF with a 60 kDa alpha and 30 kDa beta chain. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET. HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis. It can also alter epithelium morphology by the induction of nectin-1 alpha ectodomain shedding, an adhesion protein component of adherens junctions. HGF regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signalingcascade after binding to the proto oncogenic c-Met receptor. HGF is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration.
NOTE:The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
