Known As:Leukemia Inhibitory Factor; LIF; Differentiation-Stimulating Factor; D Factor; Melanoma-Derived LPL Inhibitor; MLPLI; Emfilermin; LIF; HILDA
PROPERTIES
Description:Recombinant Human Leukemia Inhibitory Factor is produced by our E.coli expression system and the target gene encoding Ser23-Phe202 is expressed.
Accession:P15018
Formulation:Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Size:10µg/50µg/500µg/1mg
Purity:> 95%
Endotoxin:< 0.01 EU/µg as determined by LAL test.
Predicted Mol Mass:19.7 KDa
Apparent Mol Mass:18 KDa, reducing conditions
Reconstitution:Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Shipping:The product is shipped at ambient temperature.
Upon receipt, store it immediately at the temperature listed below.
Storage:Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months..
Background:Leukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term
maintenance of embryonic stem cells by suppressing spontaneous differentia-tion. LIF has a number of other activities including cholinergic neuron different-iation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte produc-tion in vivo. Human and murine mature LIF exhibit a 78% sequence identity at the amino acid level. Human LIF is equally active on human and mouse cells. Murine LIF is approximately 1000 fold less active on human cells than human LIF.
NOTE:The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
