Known As: Noggin; NOG

PROPERTIES

Description:Recombinant Human Noggin is produced by our Mammalian expression system and the target gene encoding Gln28-Cys232 is expressed.

Accession:Q13253

Formulation:Lyophilized from a 0.2μm filtered solution of 20mM PB, 500mM NaCl, 2mM EDTA, pH 7.4.

Size:10µg/50µg/500µg/1mg

Purity:> 95%

Endotoxin:< 0.01 EU/µg as determined by LAL test.

Predicted Mol Mass:23 KDa

Apparent Mol Mass:25-33 KDa, reducing conditions

Reconstitution:Always centrifuge tubes before opening.Do not mix by vortex or pipetting.                       

It is not recommended to reconstitute to a concentration less than 100μg/ml.

Dissolve the lyophilized protein in distilled water.

Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Shipping:The product is shipped at ambient temperature.

Upon receipt, store it immediately at the temperature listed below.

Storage:Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.

Reconstituted protein solution can be stored at 2-8°C for 2-7 days.

Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Background:Noggin is a secreted homodimeric glycoprotein that is an antagonist of bone

morphogenetic proteins (BMPs). Mature Human Noggin contains an N-terminal acidic region, a central basic heparin-binding segment and a C-terminal cysteine-knot structure. Noggin is very highly conserved among vertebrates, such that mature human Noggin shares 99%, 99%, 98%, 97% and 89% aa sequence identity with mouse, rat bovine, equine and chicken Noggin, respectively. Secreted Noggin

probably remains close to the cell surface due to its binding of heparin-containing

proteoglycans. Noggin binds some BMPs such as BMP4 with high affinity and others such as BMP7 with lower affinity. It antagonizes BMP bioactivities by blocking epitopes on BMPs that are needed for binding to both type I and type II receptors. Noggin is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle and skin. During culture of human embryonic stem cells (hESC) or neural stem cells under certain conditions, addition of Noggin to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state, or alternatively, to differentiate into dopaminergic neurons,

NOTE:The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.