Known As:Thrombopoietin; C-mpl ligand; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; Myeloproliferative leukemia virus oncogene ligand; THPO

PROPERTIES

Description：Recombinant Mouse Thrombopoietin is produced by our Mammalian expression

system and the target gene encoding Ser22Thr356 is expressed with a 6His tag at the N-terminus.

Accession：P40226

Formulation：Lyophilized from a 0.2 μm filtered solution of PBS, 2mM EDTA, pH 7.4.

Size：10µg/50µg/500µg/1mg

Purity：> 95%

Endotoxin：< 1EU/µg as determined by LAL test.

Predicted Mol Mass：36.4 KDa

Apparent Mol Mass：65-105 KDa, reducing conditions

Reconstitution：Always centrifuge tubes before opening.Do not mix by vortex or pipetting.

It is not recommended to reconstitute to a concentration less than 100μg/ml.

Dissolve the lyophilized protein in distilled water.

Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Shipping：The product is shipped at ambient temperature.

Upon receipt, store it immediately at the temperature listed below.

Storage：Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.

Reconstituted protein solution can be stored at 2-8°C for 2-7 days.

Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Background：Thrombopoietin (TPO) is a glycoprotein hormone which belongs to the EPO/ TPO family. It produced by the liver and kidney which regulates the production of platelets.Mature mouse Tpo shares 71% and 81% aa sequence homology with human and rat Tpo, respectively. It is an 80-85 kDa protein that consists of an N-terminal domain with homology to Erythropoietin (Epo) and a C-terminal domain that contains multiple N-linked and O-linked glycosylation sites. TPO stimulates the production and differentiation of megakaryocytes, the bone marrow cells that bud off large numbers of platelets. Lineage-specific cytokine affects the prolifer-ation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage ofmegakaryocyte development. It may be the major physiolo-gical regulator of circulating platelets.

NOTE:The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.